Ashi

Ashi was the former leader and the only confirmed living member of the Daughters of Aku, seven sisters who served as assassins attempting to kill Jack. It is only later that she came to have doubts about Aku's true nature after Jack saves her life and she makes a full turn-around when Jack shows her the destruction Aku has unleashed on the world. She served as Jack's most important ally. She and Jack eventually fall in love with each other. Eventually, after meeting Aku in person, it is revealed that Aku was her biological father, after which he corrupts her unwillingly into his service.

Appearance
Ashi and all the Daughters of Aku shared the same appearance. They all had slim, petite builds and all of them have large, narrow eyes, red lips, and are 'wearing' some sort of black bodysuit. When undercover, they usually wear a white, horned mask to conceal their own identities. It is suggested they are of Asian descent.

The only difference between Ashi and her sisters is their varying hairstyles. Ashi's hair is combed up to a single point initially, though she later re-styles it when she changes her outfit.

She may be considered attractive, as the Dominator refers to her as pretty. When Jack notices her new looks, he awkwardly compliments her hair and clothing, much to her amusement.

Ashi's bodysuit is revealed to be dark magic, received when she was young after her mother pushed her (and presumably her sisters,) into a pit of burning hot dark magic from the Pit of Hate. Ashi's mother refers to the magic as the "darkness that Aku was born from." The suit was removed by Ashi while searching for Jack. Ashi now wears a fluorescent dress made from leaves, and has let her hair down in a natural style.

Ashi finds a blue-green, sweater-like uniform and black boots inside the ship. Later, during her unwilling duel against Jack, Aku provides her with a new bodysuit, including a black mask with antlers, white eyes and red flaming eyebrows, granting her abilities similar to her father.

Personality
Ashi seemed to possess a curiosity about the outside world, especially when she was younger, and developed into a cunning strategist. She was clever enough to go after the archers first in her final test, making the task much easier. Like the rest of her sisters, she possess little to no knowledge of the outside world in which she doesn't know what a deer is and assumes a male deer is Aku's minion due to its antlers. When she begins to question if Jack is truly evil, she demands he teach her and becomes impatient when he tells her to wait until the next morning. She also asks him questions like where the stars come from, further implying her love for nature and her abundant curiosity. A hallucination of her mother once referred to her as weak because she was often distracted and unfocused.

She also doesn't comprehend the sense of decency, as when her clothing was eaten by Lazarus 92 leaving her naked once more, she doesn't mind and was confused by Jack's embarrassment seeing her in such condition. She also find Jack's reminder that male and female private parts are personal does not make sense. Jack presumably taught her several concepts and terms during their relationship afterwards, as she understand the concept of marriage and living her role as Jack's wife.

Furthermore, she initially does not understand the concept of love and compassion, initially seeing the loving bond between a deer couple as madness due to the fact that she (like her sisters) assumed the stag will devour the doe. This was also seen when she and her sisters did not mourn the first of their sisters to die by Jack's hand, reciting their mother's words "death is failure". In addition, like Jack, she tends to suffer from her conscience haunting her (in the form of her mother), reminding her to kill the samurai. This part of her conscience seems to stem from the abuse she received from her mother along with being indoctrinated since birth to kill the samurai.

Still, she has an emotional breakdown after witnessing all of her sisters die before her and failing to kill Jack. This is likely due to her being raised to kill Jack under the extreme and violent tutelage of her mother, who raised them to think of failure as the pinnacle of disgrace and something that was an impossible occurrence. Her breakdown also likely stemmed from her shock at being defeated by Jack, someone she was raised to see herself and her sisters as inherently superior to.

She cares about Jack so much that she was willing to search for him and help him get get his confidence back. (implying she is prideful and a little arrogant, which could have partially led to her downfall).